Amino acid sequences of Clostridium pasteurianum flavodoxin.
نویسندگان
چکیده
Lys 10 10 8 4 8 7 5 12 9 13 9 Try 4 4 4 1 2 1 6 3 4 3 4 6 His 0 0 0 1 0 3 I 4 1 0 0 Arg 2 2 2 6 3 3 4 4 8 6 2 Asp 18 17 16 20 17 1 3 1 4 22 28 25 21 28 Thr 4 5 8 6 9 6 8 14 16 8 8 Ser 14 8 7 7 8 4 4 11 11 15 12 Glu 19 21 18 17 18 1 6 1 7 25 22 16 23 26 Pro 4 4 5 4 6 3 4 5 7 5 3 Gly 17 14 14 19 14 11 19 25 28 21 19 Ala 14 6 18 18 15 11 15 24 20 15 12 CvS 1 3 2 5 5 3 2 5 2 1 1 Val 15 10 13 10 16 5 6 17 15 10 11 Met 4 5 5 0 2 1 1 4 2 1 1 l ie 5 15 5 9 5 9 12 9 10 6 13 Leu 12 8 7 13 14 10 12 18 22 20 13 Tyr 2 3 2 5 5 4 7 5 9 5 8 Phe 3 5 4 6 3 5 9 5 6 13 9 Total 148 140 138 152 149 122 154 216 210 187 154 Molweight 1460
منابع مشابه
Efficiency of ferredoxins and flavodoxins as mediators in systems for hydrogen evolution.
1. The efficiencies of ferredoxins and flavodoxins from a range of sources as mediators in systems for hydrogen evolution were assessed. 2. In supporting electron transfer from dithionite to hydrogenase of the bacterium Clostridium pasteurianum, highest activity was shown by the ferredoxin from the cyanobacterium Chlorogloeopsis fritschii and flavodoxin from the bacterium Megasphaera elsdenii. ...
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The structural gene (nifH1) encoding the nitrogenase iron protein of Clostridium pasteurianum has been cloned and sequenced. It is located on a 4-kilobase EcoRI fragment (cloned into pBR325) that also contains a portion of nifD and another nifH-like sequence (nifH2). C. pasteurianum nifH1 encodes a polypeptide (273 amino acids) identical to that of the isolated iron protein, indicating that the...
متن کاملCloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum.
L. Sibold, M. Henriquet, O. Possot, and J.-P. Aubert (Res. Microbiol. 142:5-12, 1991) cloned and sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences of the nifH ORFs from M. barkeri showed that nifH1 clusters with nifH genes from alternative nitrogenases, while nifH2 clusters with nifH1 from the gram...
متن کاملNucleotide and deduced amino acid sequences of nifE from Clostridium pasteurianum.
We have cloned and sequenced a DNA fragment down stream from the nifHIDK operon (1) from C. pasteurianum W5. The deduced amino acids of an ORF in this fragment of DNA shares sequence similarity of 33% with that of nifE gene from Azotobacter vinelandii (2). Therefore we designate this gene as nifE of C. pasteurianum. The identical amino acids are underlined in the figure. There was no other codi...
متن کاملA hybrid Azotobacter vinelandii-Clostridium pasteurianum nitrogenase iron protein that has in vivo and in vitro catalytic activity.
Site-directed mutagenesis and gene replacement procedures were used to construct a mutant strain of Azotobacter vinelandii which expresses a hybrid nitrogenase Fe protein. This hybrid Fe protein has its carboxyl-terminal 18 residues replaced with the 5 analogous residues from the Clostridium pasteurianum Fe protein sequence. The hybrid Fe protein is 13 amino acids smaller than the wild-type A. ...
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie
دوره 27 9 شماره
صفحات -
تاریخ انتشار 1972